NANOGREEN - CHEM - (BIO) - MED

Applied Chemistry and (Bio) Medicine

NANOGREEN - CHEM - (BIO) - MED

Design and synthesis of nano-structured materials and nano-composites; preparation and surface modification of bio-materials; immobilization of active chemical species and drugs onto nano-structured supports; physico-chemical characterization of materials and their application in green chemistry and (bio) medicine, with a particular focus on antimicrobial properties and anticancer activity potential of newly developed materials and chemical species.

Optimized nano-structured multifunctional materials and processes, applications in green chemistry and medicine,
antimicrobial and anticancer activity

Synthesis of optimized multi-functional nano-materials; development and optimization of hydrothermal synthesis processes; application of dip-coating and spin-coating techniques in materials fabrication; use of electro-phoretic deposition in material preparation; chemical modification of bio-materials; comprehensive physico-chemical characterization of materials; and their application in various fields, including sorption processes and heterogeneous catalysis aimed at green chemistry and sustainable development.

Additional expertise includes applications in (bio) medicine, such as evaluation of antimicrobial activity of nano-materials and synthesized drugs against selected bacterial strains, as well as investigation of the anticancer potential of newly designed platinum- and palladium-based chemical species immobilized onto nano-structured supports, studied through both in vitro and in vivo experimental systems.

Conventional methods for the treatment of polluted water, air, and soil are often insufficient in terms of efficiency, sustainability, and cost-effectiveness. Therefore, unconventional and innovative technological solutions based on green chemistry principles are essential for achieving sustainable development. It is widely recognized that the development of new technologies is intrinsically linked to the design and implementation of new materials.

Selected nano-metric oxides and sulfides occupy an important position among advanced nano-materials due to their favorable chemical and thermal stability, durability, and low toxicity. The increasing use of nano-metric oxides in various applications necessitates further optimization of synthesis procedures as well as a deeper understanding of their functional potential.

Nano-composite materials are particularly attractive from both scientific and practical perspectives. Nano-composites exhibiting well-defined structure, desirable physico-chemical properties, and adequate resistance represent promising candidates for applications in green chemistry processes.

The synthesis of selected multi-functional nano-structured materials can be achieved through an improved one-step hydrothermal process under optimized temperature and pressure conditions and controlled gas atmosphere. Furthermore, advanced thin films and nano-material coatings can be fabricated using dip-coating and/or spin-coating techniques under carefully optimized parameters, including humidity, deposition rate, and rotational speed, temperature, and gas atmosphere.

An electro-chemical approach will be employed as a versatile and sustainable strategy for the synthesis and surface engineering of nano-structured materials and nano-composites. Controlled electro-deposition processes, conducted using a standard potentiostat, will enable precise regulation of nucleation, growth kinetics, morphology, and thickness of functional inorganic layers through adjustment of electro-chemical parameters such as applied potential, current density, and deposition time. In parallel, electro-phoretic deposition (EPD), driven by a low-voltage DC power supply, will be applied for the fabrication of uniform and adherent coatings from stable colloidal suspensions of nano-particles and hybrid systems. The combination of electro-deposition and EPD allows the preparation of multi-functional coatings at low temperatures, compatible with sensitive substrates and bio-materials, while minimizing the use of hazardous reagents in accordance with green chemistry principles.

Gradual modulation of composition, thickness, or porosity across the deposited layers allows the design of materials with spatially tailored physico-chemical and biological functionalities. In addition, the use of a potentiostat provides a powerful platform for probing the redox behaviour and electro-chemical characteristics of selected chemical species and bio-molecules.

In situ electro-chemical techniques, including cyclic voltammetry and chronoamperometry, will be used to monitor and optimize material formation and interfacial properties. This electro-chemical framework is experimentally feasible under standard laboratory conditions and provides a scalable platform for producing functional materials tailored for applications in green chemistry and (bio) medicine.

Complete physico-chemical characterization of the synthesized nano-materials will be performed, including analysis of pore structure and pore size distribution, textural properties, crystal structure and phase composition, thermal stability, morphology, surface characteristics, and electro-chemical behaviour. Characterization techniques will include BET surface area analysis, X-ray diffraction (XRD), thermogravimetric and differential thermal analysis (TG–DTA), scanning and transmission electron microscopy (SEM, TEM), Fourier-transform infrared spectroscopy (FTIR), and cyclic voltammetry. Selected characterization methods will be conducted in situ where applicable.

Planned applications in green chemistry aimed at sustainable development will involve evaluation of the synthesized materials in heterogeneous catalysis, adsorption processes, and combined catalytic–adsorptive systems. Optimization of key operational and material-related parameters will be systematically pursued in order to maximize process efficiency and sustainability.

In parallel, modern nano-composite materials are inherently multi-functional, and one of their most important application domains is (bio) medicine. Accordingly, the biomedical potential of selected nano-materials will be thoroughly investigated.

The antimicrobial activity of selected nano-materials and chemical species immobilized onto nano-structured supports will be evaluated against representative Gram-positive bacteria, Gram-negative bacteria, and fungi, using a modified standard test methods.

The evaluation of the anticancer potential of newly designed platinum and palladium complexes will be conducted through a multi-tiered experimental strategy that integrates in vitro cytotoxicity profiling, mechanistic analysis of cell death, and in vivo validation in murine tumor models. This approach is intended to characterize not only the direct effects of the complexes on tumor cell survival but also their influence on tumor progression, immune cell infiltration, and systemic cytokine signaling. All in vitro analyses will utilize authenticated human and murine cancer cell lines representing breast cancer, lung cancer, colorectal cancer, urogenital cancers and melanoma, selected to cover a spectrum of distinct tumor pathologies and to parallel the malignant conditions modeled in vivo.

In vitro cytotoxicity will first be assessed using viability- and membrane-integrity-based assays to determine dose–response characteristics and establish comparative potency profiles for the metal complexes. The MTT assay will be used as a primary measure of metabolic activity, serving as an indicator of overall cell viability following compound exposure. In parallel, the neutral red uptake assay will provide a complementary indicator of lysosomal integrity, enabling differentiation between early metabolic disturbances and subsequent organelle damage. Membrane integrity will be further evaluated using the lactate dehydrogenase (LDH) release assay, in which elevated extracellular LDH reflects loss of plasma membrane stability and progression to late-stage cytotoxicity. To elucidate the mode of cell death, Annexin V/propidium iodide (Annexin V/PI) flow cytometric analysis will be performed, allowing discrimination among viable, early apoptotic, late apoptotic, and necrotic cell populations. Together, these in vitro assays will enable the determination of concentration ranges that exert measurable biological effects, and these ranges will guide dose selection for in vivo experimentation.

In vivo antitumor efficacy will be evaluated in murine models of breast cancer, lung cancer, colorectal cancer, and melanoma. Tumor-bearing mice will receive systemic administration of doses extrapolated from in vitro findings, with adjustments made to ensure tolerability according to pilot observations. Tumor growth and progression will be monitored throughout the study period using serial volumetric measurements or imaging techniques appropriate to the specific tumor model. At the study endpoints, tumors will be collected for detailed histological examination to assess the extent of tumor cell death, tissue remodeling, vascular changes, and morphological alterations associated with treatment. In addition, the phenotype and functional characteristics of tumor-infiltrating immune cells will be examined using immunological assays such as multicolor flow cytometry or immunohistochemically analysis. These evaluations will quantify key immune cell subsets, including T lymphocyte populations, macrophage subsets, and dendritic cells, and will assess markers of activation, exhaustion, or immunosuppressive activity to better understand the immunological consequences of treatment.

To complement the tissue-level analyses, systemic immune responses will be assessed by measuring serum cytokine profiles. Serum samples collected from experimental mice will be analyzed using enzyme-linked immunosorbent assay (ELISA) methods to quantify cytokines with known roles in tumor biology. The assessed cytokines will include pro-tumorigenic mediators associated with inflammation-driven tumor progression as well as tumor-suppressive cytokines that contribute to the suppression of antitumor immunity and/or progression of apoptotic processes. Patterns of cytokine expression will be compared across treatment and control groups to determine whether the platinum or palladium complexes alter systemic cytokine dynamics in a manner consistent with their antitumor activity. Integrating these cytokine data with tumor growth metrics, histological findings, and immune infiltration patterns will support a comprehensive understanding of how these complexes influence both tumor cells and the host immune environment.

Together, the combined results of the in vitro and in vivo experiments will provide a detailed evaluation of the anticancer potential of the newly developed platinum and palladium complexes. Correlating cellular-level effects with organism-level outcomes will enable the identification of mechanistic pathways underlying the therapeutic activity of newly synthesized, selected complexes and will inform future optimization efforts aimed at improving their efficacy and safety.

• 1. ERASMUS PLUS Key Action 1 – KA 107, International Credit Mobility for Higher Education Students and Staff, Leipzig University and University of Nis (2016-2018) -(cooperation - up today); (cooperation in synthesis of new nano-materials and nanocomposites, characterization of these materials and application in environmental protection, selected industrials processses of hydrogen and biofuels production, antimicrobial activity of the selected materials)
• 2. Structure - Activity Correlation of Designed Modified Titania for Heterogeneous Photocatalytic Degradation Processes - A/10/05029, section: 324 (2010) Project funder and holder: Deutcher Akademischer Austauchdienst – DAAD
• 3. TEMPUS ISIS, JP 510985-2010: Improvement of Students Internship in Serbia (2010–2013) 4. TEMPUS WIMB, 543898-2013 WIMB: Development of Sustainable Interrelations between Education, Research and Innovation at WBC Universities in Nanotechnologies and AdvancedMaterials where Innovation Means Business (2013-2016)
• 5. DAAD, International Masters and Postgraduate Programme in Materials Science and Catalysis – MatCatNet (2013-2017) 6. Participation at ERASMUS PLUS Key Action 2, NETCHEM, ICT Networking for Overcoming Technical And Social Barriers in Instrumental Analytical Chemistry Education (2017-2019) 7. Participation at Re@WBC Enhancement of HE Research Potential Contributing to Further Growth of the WB Region, Training for young researchers in various skills
• 8. Participation at Erasmus+ Programme Key Action 1, Inter-institutional agreement between University of Nis and Babes-Bolyai University 9. Swiss National foundation SCOPES Joint Research Project “Role of Blood flow and SDF-1/CXCR4 induced recruitment of mononuclear cells in intussusceptive angiogenesis” 2014-2018 now
• 10. ,,Replica” project: multilater research project between Italy, USA, Russia, Oman, Greece and Serbia, funded by Foundation for a smoke free world 2019-till now 11. „The role of Il-33/ST2pathway in bone healing: research bilateral project between Germany and Serbia 2020-till now
• 12. FP7 project entitled: ,,Center for preclinical testing of active substances-CPCTAS” funded by EU 2008-2011

• Ossila dip-coater, Ossila spin-coater, multiple solar and UV- reactor, the autoclave(s) (different types), potentiostat, XRD (Rigaku Ultima IV, available), SEM (TESCAN Vega TS 5130 MM coupled with EDS (INCA 350)), available), FESEM (TESCAN Mira3 XMU, available), FTIR (Nicolet IS 50 FTIR spectrometer, available), The complete equipment for antimicrobial and anticancer activity (available)

• 1. Zarubica, A., Sredojević, D., Ljupković, R., Ranđelović, M., Murafa, N., Stoiljković, M., Lazić, V.,& Nedeljković, J., Photocatalytic ability of visible-light-responsive hybrid ZrO2 particles, SUSTAINABLE ENERGY and FUELS, (2023), 7(9), 2279-2287 https://doi.org/10.1039/D3SE00175J
• 2. Zarubica, A., Ljupkovic, R., Papan J., Vukoje I., Porobic S., Ahrenkiel P., Nedeljkovic J., Visible-light-responsive Al2O3 powder: Photocatalytic study, OPTICAL MATERIALS, (2020), 106, 110013 https://doi.org/10.1016/j.optmat.2020.110013
• 3. Ranđelović, M.,Momčilović, M., Purenović, J., Dornberg, G., Barascu, A., Enke, D., Zarubica, A., Exploring electrochemical and sorptive aspects of interaction between dissolved sulfides and novel Fe-enriched aluminosilicate composites, COLLOIDS and SURFACES A, (2018), 549, 196-204 https://doi.org/10.1016/j.colsurfa.2018.04.014
• 4. Ljupković, R., Stepić, K., Zarubica, A., Graphene Derivatives from Graphite Modified by Metal Oxides for Photocatalytic Usage. In: Naseer, M.N., Ikram, M., Zaidi, A.A., Abdul Wahab, Y., Johan, M.R. (eds), Graphene-Based Photocatalysts for Hydrogen Production and Environmental Remediation, ADVANCED STRUCTURED MATERIALS, (2024), 219. Springer, Cham. https://doi.org/10.1007/978-3-031-68464-7_6
• 5. Butulija, S., Valenta-Sobot, A., Todorovic, B., Petrovic, S., Radovanovic, Z., Ilic, B., Matovic, B. , Mihailovic, R., Zarubica, A., Zmejkoski, D., Filipovic-Trickovic, J., Exploring the antimicrobial and antioxidant potential of bacterial cellulose-cerium oxide nanoparticles hydrogel: Design, characterization and biomedical properties, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, (2024), 276(1), 133702 https://doi.org/10.1016/j.ijbiomac.2024.133702
• 6. Živanović AS, Bukonjić AM, Jovanović-Stević S, Bogojeski J, Ćoćić D, Bijelić AP, Ratković ZR, Volarević V, Miloradović D, Tomović DL, Radić GP. Complexes of copper(II) with tetradentate S,O-ligands: Synthesis, characterization, DNA/albumin interactions, molecular docking simulations and antitumor activity. J Inorg Biochem. 2022 Aug; 233:111861. doi: 10.1016/j.jinorgbio.2022.111861.
• 7. Drweesh EA, Kuchárová V, Volarevic V, Miloradovic D, Ilic A, Radojević ID, Raković IR, Smolková R, Vilková M, Sabolová D, Elnagar MM, Potočňák I. Low-dimensional compounds containing bioactive ligands. Part XVII: Synthesis, structural, spectral and biological properties of hybrid organic-inorganic complexes based on [PdCl4]2- with derivatives of 8-hydroxyquinolinium. J Inorg Biochem. 2022 Mar;228:111697. doi: 10.1016/j.jinorgbio.2021.111697.
• 8. Harrell CR, Volarevic A, Djonov VG, Jovicic N, Volarevic V. Mesenchymal Stem Cell: A Friend or Foe in Anti-Tumor Immunity. Int J Mol Sci. 2021 Nov 18;22(22):12429. doi: 10.3390/ijms222212429.
• 9. Trappetti V, Fazzari JM, Fernandez-Palomo C, Scheidegger M, Volarevic V, Martin OA, Djonov VG. Microbeam Radiotherapy-A Novel Therapeutic Approach to Overcome Radioresistance and Enhance Anti-Tumour Response in Melanoma. Int J Mol Sci. 2021 Jul 20;22(14):7755. doi: 10.3390/ijms22147755.
• 10. Miloradovic D, Miloradovic D, Markovic BS, Acovic A, Harrell CR, Djonov V, Arsenijevic N, Volarevic V. The Effects of Mesenchymal Stem Cells on Antimelanoma Immunity Depend on the Timing of Their Administration. Stem Cells Int. 2020 Jul 10;2020:8842659.
• 11. Jovanovic, D., Schoen, J.C., Zagorac, D., Zarubica, A., Matovic, B., Zagorac, J., Energy Landscape of Relaxation and Interaction of an Amino Acid, Glutamine (L), on Pristine and Au/Ag/Cu-Doped TiO2 Surfaces, NANOMATERIALS, (2023), 13(19), 2688 https://doi.org/10.3390/nano13192688
• 12. Marinković, M., Waisi, H., Blagojević, S., Zarubica, A., Ljupković, R., Krstić, A., Janković, B., The effect of process parameters and catalyst support preparation methods on the catalytic efficiency in transesterification of sunflower oil over heterogeneous KI/Al2O3-based catalysts for biodiesel production, FUEL, (2022), 315, 123246 https://doi.org/10.1016/j.fuel.2022.123246
• 13. Čović, J., Mirceski, V., Zarubica, A., Enke, D., Carstens, S., Bojic, A., Randjelovic, M., Palladium-graphene hybrid as an electrocatalyst for hydrogen peroxide reduction, APPLIED SURFACE SCIENCE, (2022), 574, 151633 https://doi.org/10.1016/j.apsusc.2021.151633
• 14. Butulija, S., Spasojevic, V. , Brankovic, G., Prekajski-Djordjevic, M., Radulovic, T., Zarubica, A., Matovic, B., Synthesis, characterization and magnetic properties of spider silk coated with maghemite (gamma-Fe2O3) nanoparticles, MATERIALS LETTERS, (2022), 314, 131856 https://doi.org/10.1016/j.matlet.2022.131856
• 15. Milicevic, J., Randjelovic, M., Momcilovic, M., Zarubica, A., Mofarah, S., Matovic, B., Sorrel, C., Multiwalled carbon nanotubes modified with MoO2 nanoparticles for voltammetric determination of the pesticide oxyfluorfen, MICROCHIMICA ACTA, (2020), 187(8), 429 https://doi.org/10.1007/s00604-020-04406-4
• 16. Potez M, Fernandez-Palomo C, Bouchet A, Trappetti V, Donzelli M, Krisch M, Laissue J, Volarevic V, Djonov V. Synchrotron microbeam radiation therapy as a new approach for treatment of radio-resistant melanoma: potential underlying mechanisms. Int J Radiat Oncol Biol Phys. 2019 Aug 25. pii: S0360-3016(19)33662-4. doi: 10.1016/j.ijrobp.2019.08.027.
• 17. Volarevic V, Djokovic B, Jankovic MG, Harrell CR, Fellabaum C, Djonov V, Arsenijevic N. Molecular mechanisms of cisplatin-induced nephrotoxicity: a balance on the knife edge between renoprotection and tumor toxicity. J Biomed Sci. 2019 Mar 13;26(1):25.
• 18. Volarevic V, Lako M, Erceg S, Stojkovic M. Stem Cell-Based Therapy in Transplantation and Immune-Mediated Diseases. Stem Cells Int. 2017;2017:7379136.
• 19. Gazdic M, Simovic Markovic B, Jovicic N, Misirkic-Marjanovic M, Djonov V, Jakovljevic V, Arsenijevic N, Lukic ML, Volarevic V. Mesenchymal Stem Cells Promote Metastasis of Lung Cancer Cells by Downregulating Systemic Antitumor Immune Response. Stem Cells Int 2017;2017:6294717.
• 20. Arsenijevic M, Milovanovic M, Jovanovic S, Arsenijevic N, Markovic BS, Gazdic M, Volarevic V. In vitro and in vivo anti-tumor effects of selected platinum(IV) and dinuclear platinum(II) complexes against lung cancer cells. J Biol Inorg Chem. 2017 2017; 22:807-817.
• 21. Zaheer, M., Poppitz, D., Feyzullayeva, K., Wenzel, M., Matysik, J., Ljupkovic, R. , Zarubica, A., Karavaev, A., Poeppl, A., Glaeser, R., Dvoyashkin, M., Synthesis of highly active ETS-10-based titanosilicate for heterogeneously catalyzed transesterification of triglycerides, BEILSTEIN JOURNAL OF NANOTECHNOLOGY, (2019), 10, 2039-2061 https://doi.org/10.3762/bjnano.10.200
• 22. Randjelovic, M., Momcilovic M., Milicevic, J., Djurovic-Pejcev, R., Mofarah, S.,Sorrel, C., Voltammetric sensor based on Pt nanoparticles suported MWCNT for determination of pesticide clomazone in water samples, JOURNAL of the TAIWAN INSTITUTE of CHEMICAL ENGINEERS, (2019), 105, 115-123 https://doi.org/10.1016/j.jtice.2019.10.013
• 23. Randjelovic, M., Momcilovic M., Enke, D., Mirceski, V., Electrochemistry of hydrogen peroxide reduction reaction on carbon paste electrodes modified by Ag- and Pt-supported carbon microspheres, JOURNAL of SOLID STATE ELECTROCHEMISTRY, (2019), 23(4), 1257-1267 https://doi.org/10.1007/s10008-019-04226-4
• 24. Ranđelović, M., Momčilović, M., J. Purenović, G. Dornberg, A. Barascu, D. Enke, A. Zarubica, Exploring electrochemical and sorptive aspects of interaction between dissolved sulfides and novel Fe-enriched aluminosilicate composites, COLLOIDS and SURFACES A, (2018), 549, 196-204. https://doi.org/10.1016/j.colsurfa.2018.04.014
• 25. Momcilovic, M., Randjelovic, M., Purenovic, M., Djordjevic, J., Onjia, A., Matovic, B., Morpho-structural, adsorption and electrochemical characteristics of serpentinite, SEPARATION and PURIFICATION TECHNOLOGY, (2016), 163, 72-78 https://doi.org/10.1016/j.seppur.2016.02.042
• 26. Potočňák I, Vranec P, Farkasová V, Sabolová D, Vataščinová M, Kudláčová J, Radojević ID, Čomić LR, Markovic BS, Volarevic V, Arsenijevic N, Trifunović SR. Low-dimensional compounds containing bioactive ligands. Part VI: Synthesis, structures, in vitro DNA binding, antimicrobial and anticancer properties of first row transition metal complexes with 5-chloro-quinolin-8-ol. J Inorg Biochem 2016; 154:67-77.
• 27. Mijajlovic MZ, Nikolic MV, Jevtic VV, Ratkovic ZR, Simovic Markovic B, Volarevic V, Arsenijevic NN, Novakovic SB, Bogdanovic GA, Trifunovic SR, Radic GR. Cytotoxicity of palladium (II) complexes with some alkyl derivates of thiosalicylic acid. Crystal structure of the bis (S-butylthiosalicylate) palladium (II) complex, [Pd(S-bu-thiosal)2]. Polyhedron 2015; 90:34–40.
• 28. Ljujic B, Milovanovic M, Volarevic V, Murray B, Bugarski D, Przyborski S, Arsenijevic N, Lukic ML, Stojkovic M. Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice. Sci Rep 2013; 3:2298. doi: 10.1038/srep02298.
• 29. D.P. Dimitrijević, V.V. Glođović, G.P. Radić, S. García-Granda, L. Menéndez-Taboada, M. Milovanović, V. Volarević, N. Arsenijević, G.A. Bogdanović, S.R. Trifunović. Stereospecific ligands and their complexes. Part XV. Synthesis, characterization and cytotoxicity of novel platinum (IV) complexes with some esters of ethylenediamine-N,N0-di-S,S-(2,20-dibenzyl)acetic acid. Crystal structure of O,O0-dipropyl-ethylenediamine-N,N0-di-S, S-(2,20-dibenzyl)acetate dihydrochloride. Inorganica Chimica Acta 2013; 402:83–89.
• 30. V. Volarevic, J.M. Vujic, M. Milovanovic, T. Kanjevac, A. Volarevic, S.R. Trifunovic, N. Arsenijevic. Cytotoxic effects of palladium(II) and platinum(II) complexes with O,O'-dialkyl esters of (S,S)-ethylenediamine-N,N’-di-2-(4-methyl) pentanoic acid on human colon cancer cell lines. J BUON 2013; 18(1):131- 137.
• 31. A.Volarevic, B. Ljujic, V. Volarevic, M. Milovanovic, T. Kanjevac, A. Lukic, N. Arsenijevic. A new semiquantitative method for evaluation of metastasis progression. J BUON 2012; 17:585-590.
• 32. M. Arsenijević, M. Milovanović, V. Volarević, D. Čanović, N. Arsenijević, T. Soldatović, S. Jovanović, Ž.D. Bugarčić. Cytotoxic properties of platinum(IV) and dinuclear platinum(II) complexes and their ligand substitution reactions with guanosine-50-monophosphate. Transition Met Chem 2012; 37(5):481-488.
• 33. J.M. Vujić, G.N. Kaluđerović, B.B. Zmejkovski, M. Milovanović, V. Volarević, N. Arsenijević, T.P. Stanojković, S.R. Trifunović. Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O0-dialkyl-(S,S)-ethylenediamine-N,N0- di-2-(4-methyl)pentanoate ligands. Inorganica Chimica Acta 2012; 390:123–128.
• 34. G. Radosavljevic, V. Volarevic, I. Jovanovic, M. Milovanovic, N. Pejnovic, N. Arsenijevic, D.K. Hsu, M.L. Lukic. The roles of Galectin-3 in autoimmunity and tumor progression. Immunol Res 2012; 52(1-2):100-110.
• 35. M. Milovanovic, V. Volarevic, G. Radosavljevic, I. Jovanovic, N. Pejnovic, N. Arsenijevic, M. L. Lukic. IL-33/ST2 axis in inflammation and immunopathology. Immunol Res 2012; 52(1-2):89-99.
• 36. M. Arsenijević, M. Milovanović, V. Volarević, A. Djeković, T. Kanjevac, N. Arsenijević, S. Đukić and ŽD. Bugarčić. Cytotoxicity of gold (III) complexes on A549 human lung carcinoma epithelial cell line. Med Chem 2012; 8(1):2-8(7).
• 37. Vujić JM, Kaluđerović GN, Milovanović M, Zmejkovski BB, Volarević V, Zivić D, Durđević P, Arsenijević N, Trifunović SR. Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O'-dialkyl esters of (S,S)-ethylenediamineN,N'-di-2-(4-methyl)pentanoic acid. Eur J Med Chem 2011; 46(9):4559-4565.
• 38. Volarevic V, Milovanovic M, Djekovic A, Petrovic B, Arsenijevic N, Bugarcic Z. The cytotoxic effects of some selected gold(III) complexes on 4T1 cells and their role in the prevention of breast tumor growth in BALB/c mice. J BUON 2010; 15:768-773.
• 39. Vujić JM, Cvijović M, Kaluderović GN, Milovanović M, Zmejkovski BB, Volarević V, Arsenijević N, Sabo TJ, Trifunović SR. Palladium(II) complexes with R2edda derived ligands. Part IV. O,O'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)-pentanoic acid dihydrochloride and their palladium(II) complexes: Synthesis, characterization and in vitro antitumoral activity against chronic lymphocytic leukemia (CLL) cells. Eur J Med Chem 2010; 45(9):3601- 3606.
• 40. Milovanović M, Djeković A, Volarević V, Petrović B, Arsenijević N, Bugarcić ZD. Ligand substitution reactions and cytotoxic properties of [Au(L)Cl2](+) and [AuCl2(DMSO)2]+ complexes (L=ethylenediamine and S-methyl-l-cysteine). J Inorg Biochem 2010;104(9):944-949.
• 41. Randjelovic, M., Purenovic, M., Matovic, B., Zarubica, A., Momcilovic, M., Purenovic, J., Structural, textural and adsorption characteristics of bentonite-based composite, MICRO MESOP MATER, (2014), 195, 67-74 https://doi.org/10.1016/j.micromeso.2014.03.031
• 42. Momcilovic, M., Randjelovic, M., Zarubica, A., Onjia, A., Kokunesoski, M., Matovic, B., SBA-15 templated mesoporous carbons for 2,4-dichlorophenoxyacetic acid removal, CHEMICAL ENGINEERING JOURNAL, (2013), 220, 276-283 https://doi.org/10.1016/j.cej.2012.12.024
• 43. Randjelovic, M., Purenovic, M., Zarubica, A., Purenovic, J., Matovic, B., Momcilovic, M., Synthesis of composite by application of mixed Fe, Mg (hydr)oxides coatings onto bentonite - A use for the removal of Pb(II) from water, JOURNAL of HAZARDOUS MATERIALS, (2012), 199-200, 367-374 https://doi.org/10.1016/j.jhazmat.2011.11.025
• 44. Randjelovic, M., Purenovic, M., Zarubica, A., Purenovic, J., Mladenovic, I., Nikolic, G., Alumosilicate ceramics based composite microalloyed by Sn: An interaction with ionic and colloidal forms of Mn in synthetic water, DESALINATION, (2011), 279(1-3), 353-358 https://doi.org/10.1016/j.desal.2011.06.036
• 45. Momcilovic, M., Purenovic, M., Bojic, A., Zarubica, A., Randjelovic, M., Removal of lead(II) ions from aqueous solutions by adsorption onto pine cone activated carbon, DESALINATION, (2011), 276 (1-3), 53-59 https://doi.org/10.1016/j.desal.2011.03.013
• 46. Vujicic, Dj., Comic, D., Zarubica, A., Micic, R., Boskovic, G., Kinetics of biodiesel synthesis from sunflower oil over CaO heterogeneous catalyst, FUEL, (2010), 89(8), 2054-2061 https://doi.org/10.1016/j.fuel.2009.11.043
• 47. Dinic Ljubomir A, Basic Dragoslav T, Ignjatovic Ivan M, Dinic Vesna D, Vukovic Natalija, Golubovic Mladjan D, Djordjevic Miodrag N, Laketic Darko M, Veljkovic Andrej R , The first 10 years' experience in radical retropubic prostatectomy: complications, lower urinary tract symptoms, and quality of life - a single-center experience (Article) , SRPSKI ARHIV ZA CELOKUPNO LEKARSTVO, (2021), vol. 149 br. 9-10, str. 566-572 .
• 48. Ignjatovic Ivan M, Potic Milan B, Basic Dragoslav T, Dinic Ljubomir A, Skakic Aleksandar, Laparoscopic minimally invasive sacrocolpopexy or hysteropexy and transobturator tape combined with native tissue repair of the vaginal compartments in patients with advanced pelvic organ prolapse and incontinence, INTERNATIONAL UROGYNECOLOGY JOURNAL, (2021), vol. 32 br. 4, str. 967-974 .
• 49. Golubovic Mladjan D, Peric Velimir S, Stosic Marija D, Stojiljkovic Vladimir, Kostic Tomislav L, Kamenov Aleksandar B, Lazarevic Milan V, Stojanovic Dalibor, Veselinovic Aleksandar M, Development of Novel Analgesics Related to TRPV1 Antagonism - In Silico Approach, ACTA CHIMICA SLOVENICA, (2025), vol. 72 br. 3, str. 450-462.
• 50. Peric Velimir S, Golubovic Mladjan D, Stosic Marija D, Milic Dragan J, Lazovic Lela, Stojanovic Dalibor, Lazarevic Milan V, Markovic Dejan Z, Unic-Stojanovic Dragana R, Echocardiographic Predictors of Postoperative Atrial Fibrillation After Cardiac Surgery: Assessing Atrial Mechanics for Risk Stratification, JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE, (2025), vol. 12 br. 4, str. -.

- 2013-2015 Innovation project funded by the Ministry of Science of Serbia ID 451-03-2802/2013-16/11 and industry,
Design and development of a potentiostat with electrochemical sensors for monitoring the quality of wastewater and other water sources, and for the electrochemical characterization of micro-alloyed and nanostructured materials (cooperation with industry)
- 2020 – 2022 The Proof of Concept program, Republic of Serbia Innovation Fund ID 5726 and industry,
Realization of a new measurement system for the monitoring of Pb2+ concentration in water (cooperation with industry)
- 2022-2023. Technology Transfer Program (TT Program), Republic of Serbia Innovation Fund ID 1126 Design and realization of a new measurement system based on electrochemical sensor for the monitoring Pb2+ ions in water“ (cooperation with industry)
- Philip Morris International Co.

• 1. Zarubica, A., Sredojević, D., Ljupković, R., Ranđelović, M., Murafa, N., Stoiljković, M., Lazić, V.,& Nedeljković, J., Photocatalytic ability of visible-light-responsive hybrid ZrO2 particles, SUSTAINABLE ENERGY and FUELS, (2023), 7(9), 2279-2287 https://doi.org/10.1039/D3SE00175J
• 2. Zarubica, A., Ljupkovic, R., Papan J., Vukoje I., Porobic S., Ahrenkiel P., Nedeljkovic J., Visible-light-responsive Al2O3 powder: Photocatalytic study, OPTICAL MATERIALS, (2020), 106, 110013 https://doi.org/10.1016/j.optmat.2020.110013
• 3. Ranđelović, M.,Momčilović, M., Purenović, J., Dornberg, G., Barascu, A., Enke, D., Zarubica, A., Exploring electrochemical and sorptive aspects of interaction between dissolved sulfides and novel Fe-enriched aluminosilicate composites, COLLOIDS and SURFACES A, (2018), 549, 196-204 https://doi.org/10.1016/j.colsurfa.2018.04.014
• 4. Ljupković, R., Stepić, K., Zarubica, A., Graphene Derivatives from Graphite Modified by Metal Oxides for Photocatalytic Usage. In: Naseer, M.N., Ikram, M., Zaidi, A.A., Abdul Wahab, Y., Johan, M.R. (eds), Graphene-Based Photocatalysts for Hydrogen Production and Environmental Remediation, ADVANCED STRUCTURED MATERIALS, (2024), 219. Springer, Cham. https://doi.org/10.1007/978-3-031-68464-7_6
• 5. Butulija, S., Valenta-Sobot, A., Todorovic, B., Petrovic, S., Radovanovic, Z., Ilic, B., Matovic, B. , Mihailovic, R., Zarubica, A., Zmejkoski, D., Filipovic-Trickovic, J., Exploring the antimicrobial and antioxidant potential of bacterial cellulose-cerium oxide nanoparticles hydrogel: Design, characterization and biomedical properties, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, (2024), 276(1), 133702 https://doi.org/10.1016/j.ijbiomac.2024.133702
• 6. Živanović AS, Bukonjić AM, Jovanović-Stević S, Bogojeski J, Ćoćić D, Bijelić AP, Ratković ZR, Volarević V, Miloradović D, Tomović DL, Radić GP. Complexes of copper(II) with tetradentate S,O-ligands: Synthesis, characterization, DNA/albumin interactions, molecular docking simulations and antitumor activity. J Inorg Biochem. 2022 Aug; 233:111861. doi: 10.1016/j.jinorgbio.2022.111861.
• 7. Drweesh EA, Kuchárová V, Volarevic V, Miloradovic D, Ilic A, Radojević ID, Raković IR, Smolková R, Vilková M, Sabolová D, Elnagar MM, Potočňák I. Low-dimensional compounds containing bioactive ligands. Part XVII: Synthesis, structural, spectral and biological properties of hybrid organic-inorganic complexes based on [PdCl4]2- with derivatives of 8-hydroxyquinolinium. J Inorg Biochem. 2022 Mar;228:111697. doi: 10.1016/j.jinorgbio.2021.111697.
• 8. Harrell CR, Volarevic A, Djonov VG, Jovicic N, Volarevic V. Mesenchymal Stem Cell: A Friend or Foe in Anti-Tumor Immunity. Int J Mol Sci. 2021 Nov 18;22(22):12429. doi: 10.3390/ijms222212429.
• 9. Dinic Ljubomir A, Basic Dragoslav T, Ignjatovic Ivan M, Dinic Vesna D, Vukovic Natalija, Golubovic Mladjan D, Djordjevic Miodrag N, Laketic Darko M, Veljkovic Andrej R , The first 10 years' experience in radical retropubic prostatectomy: complications, lower urinary tract symptoms, and quality of life - a single-center experience (Article) , SRPSKI ARHIV ZA CELOKUPNO LEKARSTVO, (2021), vol. 149 br. 9-10, str. 566-572 .
• 10. Peric Velimir S, Golubovic Mladjan D, Stosic Marija D, Milic Dragan J, Lazovic Lela, Stojanovic Dalibor, Lazarevic Milan V, Markovic Dejan Z, Unic-Stojanovic Dragana R, Echocardiographic Predictors of Postoperative Atrial Fibrillation After Cardiac Surgery: Assessing Atrial Mechanics for Risk Stratification, JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE, (2025), vol. 12 br. 4, str. -.
• Team references are above listed among 50 selected team publications.

Positive international cooperation of team members is above cited and proved by joint articles, publications,
grants, projects and programs.